![]() ![]() ![]() ![]() Importantly, the tree seed oils did not cause increased cell death as the number of dead cells remained unchanged under control and oil-treated conditions. All three tree seed oils suppressed both Caco-2 and HEK-293 cell growth in a dose-dependent manner. The trypan blue dye exclusion method was used to determine cell growth 48-hours after oil treatment. Human colon adenocarcinoma (Caco-2) and human embryonic kidney (HEK-293) cells were maintained and treated with various concentrations (0, 20, 40, 80, 100 and 120 mg/l) of K. In this study, we determined the effects of the Kigelia africana, the Mimusops zeyheri and the Ximenia caffra seed-oil on cell proliferation in culture. Animal-derived oils and purified fatty acids, but not indigenous fruit-tree-derived seed oils, have been used to study cell growth and differentiation. ![]()
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